CJC-1295 no DAC 10mg reconstitution takes bacteriostatic water, careful math, and sterile technique. See dilution charts, storage tips, and safety notes.
Reconstituting CJC-1295 no DAC 10mg means adding bacteriostatic water to the lyophilized powder so the peptide can be measured with a syringe. Most researchers add 1 to 3 mL of bacteriostatic water to a 10 mg vial, which produces a concentration between 3.3 mg/mL and 10 mg/mL, then swirl gently and refrigerate. CJC-1295 without DAC is sold as a research chemical and is not FDA-approved for human use in the United States.
This guide covers the supplies, the dilution math, storage, and the safety questions that come up most often with a 10 mg vial. Nothing here is medical advice, and no peptide described on this page is a treatment or cure for any condition.
What CJC-1295 No DAC Actually Is
CJC-1295 no DAC is the peptide fragment Mod GRF 1-29, a modified form of growth hormone-releasing hormone with four amino acid substitutions that slow its breakdown in the body. The no DAC label means the drug affinity complex, a maleimide group that binds to albumin, has been removed from the molecule.
That one structural change drives most of the practical differences between the two forms. The cjc-1295 dac vs no dac comparison usually comes down to half-life: the DAC version circulates for several days, while the no DAC version has a half-life measured in roughly 30 minutes.
A 10 mg vial contains lyophilized, or freeze-dried, powder. Lyophilized peptides stay stable for long periods when kept cold and dry, but they cannot be measured accurately until they are dissolved in liquid.
Supplies You Need Before You Start
Gather everything before you open the vial. A crowded workspace is how contamination and mix-ups happen.
- One 10 mg vial of lyophilized CJC-1295 no DAC
- Bacteriostatic water (0.9% benzyl alcohol), the standard diluent for multi-dose vials
- A 3 mL luer-lock syringe with an 18 to 21 gauge needle for transferring water
- Alcohol swabs and sterile gloves
- U-100 insulin syringes for measuring small volumes later
- A sharps container and a labeled storage box
Bacteriostatic water is preferred over plain sterile water because the benzyl alcohol slows bacterial growth, which matters when a single vial is punctured more than once. Plain sterile water is intended for single-use vials only.
Step-by-Step: How to Reconstitute a 10 mg Vial
Nearly every guide on cjc-1295 no dac reconstitution follows the same sequence. Slow, gentle handling protects the peptide, while vigorous shaking can damage it.
- Let the peptide vial and the bacteriostatic water reach room temperature.
- Wipe the rubber stopper on both vials with an alcohol swab and let them air dry.
- Draw your chosen volume of bacteriostatic water into the transfer syringe (1 mL, 2 mL, or 3 mL).
- Insert the needle into the peptide vial at an angle so the stream runs down the glass wall instead of hitting the powder directly.
- Remove the needle and swirl the vial gently between your fingers until the powder fully dissolves. Do not shake it.
- Inspect the solution, which should be clear and free of particles.
- Label the vial with the peptide name, the concentration, and the date.
- Store the vial upright in a refrigerator at 2 to 8 °C, away from light.
The Dilution Math for a 10 mg Vial
Ten milligrams equals 10,000 micrograms, so the final concentration depends entirely on how much water you add. The table below shows the most common options and the volume needed for a 100 mcg measurement on a U-100 insulin syringe, where 1 unit equals 0.01 mL.
| Bacteriostatic water added | Concentration | Volume for 100 mcg | Units on a U-100 syringe |
|---|---|---|---|
| 1 mL | 10 mg/mL (10,000 mcg/mL) | 0.01 mL | 1 unit |
| 2 mL | 5 mg/mL (5,000 mcg/mL) | 0.02 mL | 2 units |
| 3 mL | 3.33 mg/mL (3,333 mcg/mL) | 0.03 mL | 3 units |
| 5 mL | 2 mg/mL (2,000 mcg/mL) | 0.05 mL | 5 units |
More water makes each unit easier to measure, but it also forces a larger injection volume. Most researchers settle on 2 mL to 3 mL for a 10 mg vial because it balances accuracy against volume.
The same arithmetic applies when you are working with a cjc-1295 dac 10mg vial, since the DAC version also ships as a 10 mg lyophilized powder. What changes between the two is the timing of the research protocol, not the reconstitution math.
Storage and Stability After Reconstitution
Lyophilized powder stored in a freezer at -20 °C can remain stable for years. Once the peptide is dissolved, the clock starts.
- Refrigerate reconstituted vials at 2 to 8 °C and do not freeze them again.
- Keep vials upright, capped, and out of direct light.
- Many vendors recommend using reconstituted peptide within 4 to 8 weeks.
- Discard any solution that turns cloudy, gels, or contains floating particles.
Bacteriostatic water extends practical shelf life by suppressing microbial growth, but it does not stop peptide degradation. Write the reconstitution date on the label so you are not guessing weeks later.
Dosing, Combinations, and Legal Status
CJC-1295 no DAC is not FDA-approved for human use, and US pharmacies do not dispense it. It is sold strictly for laboratory research, which means any numbers reported in published studies are research parameters, not prescriptions.
Published papers on cjc-1295 no dac dosage typically describe microgram-level amounts given by injection, often once or twice daily, but study designs vary widely and results are not directly comparable. Many labs also track cjc-1295 no dac ipamorelin 10mg blend dosage because the two peptides are frequently studied together, even though a blend is a separate product with its own concentration and its own dilution math.
Anyone considering use in a human being should speak with a licensed healthcare professional first. Self-dosing research chemicals carries real risk of measurement errors, contamination, and unknown side effects.
Quick Safety Checklist
Sterile technique, correct math, and cold storage prevent the majority of problems people run into with peptide vials. Skipping any one of the three introduces risk that no protocol can fix.
- Never share syringes, needles, or vials between people.
- Double-check your concentration before drawing any dose.
- Source peptides only from vendors that publish third-party purity testing.
- Treat every research chemical as unproven in humans until regulators say otherwise.
Frequently Asked Questions
How much bacteriostatic water should I add to a 10 mg vial of CJC-1295 no DAC?
Most protocols add 1 to 3 mL of bacteriostatic water to a 10 mg vial. Adding 2 mL creates a 5 mg/mL solution, so a 100 mcg measurement equals 2 units on a U-100 insulin syringe. Adding more water makes small volumes easier to measure but increases the injection volume.
How long does reconstituted CJC-1295 no DAC last in the refrigerator?
Stored at 2 to 8 °C and protected from light, most vendors suggest using reconstituted peptide within 4 to 8 weeks. Freezing a reconstituted vial can damage the peptide, so it should be refrigerated, not frozen again. Discard any solution that becomes cloudy or shows particles.
Is CJC-1295 no DAC legal to buy in the United States?
It is sold as a research chemical for laboratory use and is not FDA-approved for human use. Purchasing it for personal human use falls outside that labeled purpose, and product quality varies between vendors. Anyone with a medical question should consult a licensed healthcare professional.
This page provides educational research information and does not replace medical advice, diagnosis, or treatment.