Enter stock concentration
Use a concentration measured in the same mass-per-volume unit as the target concentration.
Peptide Dilution Calculator
Calculate how much stock solution and diluent are required to prepare a lower target concentration at a chosen final volume.
LIVE CALCULATION
C1V1 = C2V2 dilution planning
Use matching concentration units for C1 and C2. The volume fields are calculated in mL.
Stock volume needed1 mL
Diluent to add4 mL
Dilution factor5×
Final material amount10 mg
1 mL stock4 mL diluent
Current equationV1 = (2 × 5) ÷ 10 = 1 mL
Simple dilution lowers concentration while preserving the amount of dissolved material.
METHOD & LIMITS
How this calculator works
Stock volume (V1) = target concentration (C2) × final volume (V2) ÷ stock concentration (C1). Diluent volume = V2 − V1.
EquationC1V1 = C2V2
OutputStock and diluent volumes
UnitsAny consistent concentration unit
ValidationRejects impossible simple dilutions
Enter target concentration
The target must be lower than or equal to the stock concentration for a simple dilution.
Enter final volume
The result separates the stock volume from the volume of diluent to add.
Research calculation notice: This page performs mathematical conversions using values entered by the reader. It does not verify a product, select an amount, define a protocol, or provide medical or veterinary advice.
Peptide Dilution Calculator FAQ
What does C1V1 = C2V2 mean?
C1 and V1 are the starting concentration and stock volume. C2 and V2 are the target concentration and final total volume. The equation conserves the amount of dissolved material.
Can I use mg/mL and mcg/mL together?
Convert both concentrations to the same unit first. The ratio is valid only when C1 and C2 use matching units.
Why must target concentration be lower than stock concentration?
Adding diluent can lower concentration but cannot create a concentration higher than the stock. A higher target requires a different stock or a concentration step.
Does final volume equal the amount of diluent added?
No. Final volume is stock volume plus diluent volume. The calculator subtracts the required stock volume to find the diluent volume.