SS-31 Dosage: What Human Trials Actually Used

There is no FDA-approved SS-31 dosage for human use. In human clinical trials, the drug form of SS-31, called elamipretide, was most often given as 40 mg injected subcutaneously once daily, while some heart-failure studies used intravenous infusions of roughly 0.05 mg/kg per hour. Research-grade SS-31 sold online has no standard dose, and self-reported amounts usually fall between about 1 mg and 10 mg per day.

What SS-31 Is and Why Its Dose Is Unsettled

SS-31 is a synthetic tetrapeptide that concentrates inside mitochondria and binds to cardiolipin, a lipid on the inner mitochondrial membrane. It is also known as elamipretide, MTP-131, and Bendavia.

Dosing information for this peptide comes from three very different sources: controlled human trials, animal studies, and anecdotal online reports. Those sources disagree with each other, which is why the search for one correct SS-31 dose produces so many conflicting answers.

SS-31 is not FDA-approved for human use in the United States, and no official dosing guideline exists.

SS-31 Doses Used in Human Clinical Trials

Clinical trials are the highest-quality source of SS-31 dosing data. Sponsors generally used a fixed 40 mg subcutaneous dose in adults without adjusting for body weight, and lower weight-based doses when the peptide was infused intravenously.

Study population	Route	Dose	Frequency
Barth syndrome (TAZPOWER)	Subcutaneous injection	40 mg	Once daily
Primary mitochondrial myopathy (MMPOWER-3)	Subcutaneous injection	40 mg	Once daily
Age-related macular degeneration (ReCLAIM)	Subcutaneous injection	40 mg	Once daily
Heart failure (intravenous studies)	IV infusion	About 0.05 mg/kg per hour	Single supervised infusion

Because the 40 mg trial dose was not weight-adjusted, a 60 kg participant and a 100 kg participant received the same amount. Intravenous protocols were also administered under clinical supervision, which is a meaningful difference from at-home subcutaneous use.

Research-Grade and Self-Reported SS-31 Dosage Ranges

Outside of trials, SS-31 is sold as a lyophilized research chemical with no dose printed on the label. Vendor guides and community discussions, including ss-31 dosage reddit threads and the ss-31 dosage jay campbell podcast coverage, describe ranges rather than one agreed number.

Reported context	Amount per day	Typical schedule	Evidence level
Lower-end self-reported	1–2 mg	Once daily	Anecdotal only
Middle-range self-reported	5 mg	Once daily, 4–8 weeks	Anecdotal only
Higher self-reported	10 mg	Once daily, cycled	Anecdotal only
Clinical trial dose	40 mg	Once daily, subcutaneous	Phase 2 and Phase 3 trials

Many people who follow a formal ss 31 dosage protocol simply copy the 40 mg trial amount, while others start far lower to gauge tolerance. Neither approach has been validated outside a clinical trial setting.

Reconstituting and Measuring an SS-31 Dose

Most SS-31 arrives as a powder that must be mixed with bacteriostatic water before use. The math matters here, because a small dilution error changes the injected amount by a large margin.

The formula is straightforward: concentration equals total peptide in milligrams divided by total water volume in milliliters. A 10 mg vial mixed with 2 mL of water gives 5 mg/mL, so 0.2 mL contains 1 mg. That same vial mixed with 1 mL gives 10 mg/mL, so 0.1 mL contains 1 mg.

Detailed ss-31 reconstitution and dosage walkthroughs from suppliers usually suggest 1–2 mL of diluent per 10 mg vial, which keeps injection volumes small and easy to draw into a U-100 insulin syringe.

Vial size	Diluent added	Concentration	Volume for 1 mg	Volume for 5 mg
10 mg	1 mL	10 mg/mL	0.10 mL	0.50 mL
10 mg	2 mL	5 mg/mL	0.20 mL	1.00 mL
50 mg	5 mL	10 mg/mL	0.10 mL	0.50 mL

Because the commonly discussed ss-31 peptide injection dosage is subcutaneous, rotate injection sites and use sterile technique. Never reuse needles or share vials between people.

Risks, Side Effects, and Legal Status

In published trials, the most frequent side effects of elamipretide were injection-site reactions such as redness, swelling, and pain. Headache, nausea, dizziness, and fatigue were also reported.

Elamipretide has not received FDA approval. The MMPOWER-3 trial in primary mitochondrial myopathy did not meet its primary endpoint, and the Barth syndrome and ophthalmology programs have run into regulatory setbacks. Even the 40 mg trial dose is therefore not a proven treatment.

Research-grade SS-31 is not verified by the FDA for identity, purity, or sterility, so the actual content of an online vial may differ from its label.

Practical Takeaways on SS-31 Dosage

• Human trials used 40 mg subcutaneously once daily, which remains the best available dose reference.
• Intravenous trial doses were weight-based at roughly 0.05 mg/kg per hour and were given under medical supervision.
• Self-reported research doses typically range from 1 mg to 10 mg per day, with no evidence that these amounts are effective or safe.
• Reconstitution math sets the real dose: 10 mg in 2 mL equals 5 mg/mL, so 0.2 mL equals 1 mg.
• SS-31 has no FDA approval, no standard dose, and no long-term human safety data.

Anyone weighing SS-31 use should treat every number on this page as a research data point, not a recommendation, and discuss it with a licensed healthcare professional first.

COMMON QUESTIONS

Frequently Asked Questions

What is the typical SS-31 dosage?

No official SS-31 dosage exists because the peptide is not FDA-approved for human use. Human trials of elamipretide used 40 mg injected subcutaneously once daily, while intravenous studies used about 0.05 mg/kg per hour. Community-reported research doses range from roughly 1 mg to 10 mg per day with no supporting evidence.

How much bacteriostatic water should I mix with SS-31?

Most 10 mg SS-31 vials are reconstituted with 1 to 2 mL of bacteriostatic water, producing either a 10 mg/mL or a 5 mg/mL solution. At those concentrations, 0.1 mL or 0.2 mL respectively contains 1 mg of peptide. Calculate your own concentration and verify it before drawing any dose.

Is SS-31 legal to buy in the United States?

SS-31 is sold as a research chemical intended for laboratory use, not as an approved drug. It is not FDA-approved for human use, so buying or possessing it for personal injection falls outside its labeled purpose. Anyone considering it should consult a licensed healthcare professional.